Publications

    1. Knapp DJ, Hammond CA, Miller PH, Rabu GM, Beer PA, Ricicova M, Lecault V, Da Costa D, VanInsberghe M, Cheung AM, Pellacani D, Piret J, Hansen C, Eaves CJ. “Dissociation of Survival, Proliferation, and State Control in Human Hematopoietic Stem Cells.” Stem Cell Reports. (2017) Jan 10;8(1):152-162
    2. Zahn H, Steif A, Laks E, Eirew P, VanInsberghe M, Shah SP, Aparicio S, Hansen CL. “Scalable whole-genome single-cell library preparation without preamplification.” Nat Methods. (2017) Feb;14(2):167-173.
    3. Knapp DJ, Hammond CA, Aghaeepour N, Miller PH, Pellacani D, Beer PA, Sachs K, Qiao W, Wang W, Humphries RK, Sauvageau G, Zandstra PW, Bendall SC, Nolan GP, Hansen C, Eaves CJ. “Distinct signaling programs control human hematopoietic stem cell survival and proliferation.” Blood. (2017) Jan 19;129(3):307-318.
    4. Krowiorz K, Ruschmann J, Lai C, Ngom M, Maetzig T, Martins V, Scheffold A, Schneider E, Pochert N, Miller C, Palmqvist L, Staffas A, Mulaw M, Bohl SR, Buske C, Heuser M, Kraus J, O’Neill K, Hansen CL, Petriv OI, Kestler H, Döhner H, Bullinger L, Döhner K, Humphries RK, Rouhi A, Kuchenbauer F. “MiR-139-5p is a potent tumor suppressor in adult acute myeloid leukemia.” Blood Cancer J. (2016) Dec 9;6(12):e508
    5. Leung K, Klaus A, Lin BK, Laks E, Biele J, Lai D, Bashashati A, Huang YF, Aniba R, Moksa M, Steif A, Mes-Masson AM, Hirst M, Shah SP, Aparicio S, Hansen CL. “Robust high-performance nanoliter-volume single-cell multiple displacement amplification on planar substrates.” Proc Natl Acad Sci U S A. (2016) Jul 26;113(30):8484-9
    6. Eirew P, Steif A, Khattra J, Ha G, Yap D, Farahani H, Gelmon K, Chia S, Mar C, Wan A, Laks E, Biele J, Shumansky K, Rosner J, McPherson A, Nielsen C, Roth AJ, Lefebvre C, Bashashati A, de Souza C, Siu C, Aniba R, Brimhall J, Oloumi A, Osako T, Bruna A, Sandoval JL, Algara T, Greenwood W, Leung K, Cheng H, Xue H, Wang Y, Lin D, Mungall AJ, Moore R, Zhao Y, Lorette J, Nguyen L, Huntsman D, Eaves CJ, Hansen C, Marra MA, Caldas C, Shah SP, Aparicio “Dynamics of genomic clones in breast cancer patient xenografts at single-cell resolution.” NATURE (2015) Feb 19;518(7539):422-6. Epub 2014 Nov 26. PMID: 25470049
    7. Sikorski D, Caron N, VanInsberghe M, Zahn H, Eaves C, Piret J, Hansen CL. “Clonal analysis of individual human embryonic stem cell differentiation patterns in microfluidic cultures.” BIOTECHNOLOGY JOURNAL (2015). Jun 9. [Epub ahead of print] PMID: 26059045
    8. Petriv OI, Heyries KA, VanInsberghe M, Walker D, Hansen CL. “Methods for multiplex template sampling in digital PCR assays.” PLOS ONE. (2014) May 22;9(5):e98341. PMID: 24854517
    9. Walsh C, Ou K, Belliveau NM, Leaver TJ, Wild AW, Huft J, Lin PJ, Chen S, Leung AK, Lee JB, Hansen CL, Taylor RJ, Ramsay EC, Cullis PR. “Microfluidic-Based Manufacture of siRNA-Lipid Nanoparticles for Therapeutic Applications.” METHODS MOL BIOL. (2014); 1141:109-20. PMID: 24567134
    10. White AK, Heyries KA, Doolin C, Vaninsberghe M, Hansen CL. “High-throughput microfluidic single-cell digital polymerase chain reaction.” ANALYTICAL CHEMISTRY (2013) Aug 6;85(15):7182-90. Epub 2013 Jul 24. PMID: 23819473
    11. Ricicova M, Hamidi M, Quiring A, Niemistö A, Emberly E, Hansen CL. “Dissecting genealogy and cell cycle as sources of cell-to-cell variability in MAPK signaling using high-throughput lineage tracking.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES (2013) Jul 9;110(28):11403-8. Epub 2013 Jun 26. PMID: 23803859
    12. Huft J, Haynes CA, Hansen CL. “Microfluidic integration of parallel solid-phase liquid chromatography.” ANALYTICAL CHEMISTRY (2013) Mar 5;85(5):2999-3005. Epub 2013 Feb 22. PMID: 23384109
    13. Huft J, Haynes CA, Hansen CL. “Fabrication of high-quality microfluidic solid phase chromatography columns”. ANALYTICAL CHEMISTRY (2013) Feb 5;85(3):1797-802. Epub 2013 Jan 10. PMID: 23234506
    14. Leung AK, Hafez IM, Baoukina S, Belliveau NM, Zhigaltsev IV, Afshinmanesh E, Tieleman DP,Hansen CL, Hope MJ, Cullis PR. “Lipid Nanoparticles Containing siRNA Synthesized by Microfluidic Mixing Exhibit an Electron-Dense Nanostructured Core”. THE JOURNAL OF PHYSICAL CHEMISTRY C Nanometer Interfaces (2012) Aug 30;116(34):18440-18450. Epub 2012 Jul 18. PMID: 22962627
    15. Reitinger S, Petriv OI, Mehr K, Hansen CL, Withers “Purification and quantitation of bacteriophage M13 using desalting spin columns and digital PCR”, JOURNAL OF VIROLOGICAL METHODS (2012) Oct;185(1):171-4. Epub 2012 Jul 2. PMID: 22766184
    16. Belliveau NM, Huft J, Lin PJ, Chen S, Leung AK, Leaver TJ, Wild AW, Lee JB, Taylor RJ, Tam YK, Hansen CL, Cullis PR. “Microfluidic Synthesis of Highly Potent Limit-Size Lipid Nanoparticles for In Vivo Delivery of siRNA”. MOLECULAR THERAPY-NUCLEIC ACIDS (2012) Aug 14;1:e37. PMID: 23344179.
    17. Sloma I, Imren S, Beer PA, Zhao Y, LecaultV, Leung D, Raghuram K, Brimacombe C, Lambie K, Piret J, Hansen C, Humphries RK, Eaves CJ. “Ex vivo expansion of normal and chronic myeloid leukemic stem cells without functional alteration using a NUP98HOXA10homeodomain fusion gene.” LEUKEMIA (2013) Jan;27(1):159-69. Epub 2012 Jul 16. PMID: 22868969.
    18. Leung, K., H. Zahn, T. Leaver, K.M. Kishori, N.W. Hanson, A.P. Pagé, C-C Lo, P.S. Chain, S. Hallam, C.L. Hansen. “A programmable droplet-based microfluidic device applied to multiparameter analysis of single microbes and microbial communities”. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES (2012) May 15;109(20):7665-70. Epub 2012 Apr 30.
    19. Lecault, V, A.K. White, A. Singhal, C.L. Hansen. “Microfluidic single cell analysis: from promise to practice”. CURRENT OPINION IN CHEMICAL BIOLOGY (2012) Apr 21. [Epub ahead of print]
    20. VanInsberghe M, K.A. Heyries, C.L. Hansen. “Should genetic testing go digital?” EXPERT REV MOL DIAGN (2012) Mar;12(2):111-4.
    21. Zhigaltsev I, N. Belliveau, I. Hafez, A.K. Leung, J. Huft, C. Hansen, P. Cullis. “Bottom-up Design and Synthesis of Limit Size Lipid Nanoparticle Systems with Aqueous and Triglyceride Cores using Millisecond Microfluidic Mixing.” LANGMUIR (2012) Feb 21;28(7):3633-40. Epub 2012 Feb 9. 
    22. Heyries, K.A. & C.L. Hansen. “Parylene C coating for improved soft lithography-based device production.” LAB ON A CHIP (2011) Nov 8;(23):4122-5. Epub 2011 Oct 13. 
    23. Sekulovic, S., M. Gasparetto, V. Lecault, C.A. Hoesli, D.G. Kent, P. Rosten, A. Wan, C. Brookes, C.L. Hansen, J.M. Piret, C. Smith, C.J. Eaves, and R.K. Humphries. “Ontogeny stage-independent and high-level clonal expansion in-vitro of mouse hematopoietic stem cells stimulated by an engineered NUP98-HOX fusion transcription factor.” BLOOD (2011) Oct 20;118(16):4366-76. Epub 2011 Aug 24. 
    24. White, A., M. VanInsberghe, O.I. Petriv, M. Hamidi, M.A. Marra, J.M. Piret, S. Aparicio, and C.L. Hansen. “High-Throughput Microfluidic RT-qPCR of Single Cells”. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES (2011) Aug 23;108(34):13999-4004. 
    25. Heyries, K.A., C. Tropini, M. VanInsberghe, O.I. Petriv, C. Hugesman, A. Singhal, K. Leung, C.L. Hansen. “Megapixel Digital PCR”. NATURE METHODS (2011) Jul 3;8(8):649-51. 
    26. Lecault, V., M. VanInsberghe, S. Sekulovic, T. McLaughlin, A. Jarandehei, M.M. Miller, F. Taghipour, D. Falconnet, A.K. White, D.G. Kent, M.R. Copley, C.J. Eaves, R.K. Humphries, J.M. Piret, C.L. Hansen. “High-Throughput Analysis of Single Hematopoietic Stem Cell Proliferation in Perfusion Microfluidic Cell Culture Arrays”, NATURE METHODS (2011) May 22. 8(7):581-6. 
    27. Petriv, O.I., C.L. Hansen, R.K. Humphries, F. Kuchenbauer. “Probing the complexity of miRNA expression across hematopoiesis”. CELL CYCLE (2011) 10(1): 15443-8. 
    28. Liu, Q., B. Larsen, M. Ricicova, S. Orlicky, H. Tekotte, X. Tang, K. Craig, A. Quiring, T. Le Bihan, C.L. Hansen, F. Sicheri, M. Tyers. “SCFCdc4 enables mating type switching in yeast by CDK-dependent elimination of the Ash1 transcriptional repressor”. MOLECULAR AND CELLULAR BIOLOGY (2011) 31(3):584-9. 
    29. Falconnet, D., Niemisto, A., Taylor, R.J., Ricicova, M., Galitski, T., Shmulevich, I., Hansen, C.L., “High-Throughput Tracking of Single Yeast Cells in a Microfluidic Imaging Matrix”. LAB ON A CHIP(2011) 11(3):466-7. 
    30. Starczynowski, D.T., F. Kuchenbauer, J. Wegrzyn, A. Rouhi, O.I. Petriv, C.L. Hansen, R.K. Humphries, A. Karsan. “MicroRNA-146a disrupts hematopoietic differentiation and survival”, EXPERIMENTAL HEMATOLOGY (2011) 39(2):167-178.e4. 
    31. Singhal, A., Haynes, C., Hansen, C.L., “Microfluidic Measurement of Antibody-Antigen Binding Kinetics from Low Abundance Samples and Single Cells”. ANALYTICAL CHEMISTRY (2010) 82 (20):8671-9.
    32. Petriv, O.I., F. Kuchenbauer, A. D. Delaney, V. Lecault, A.K. White, D. Kent, L. Marmolejo, M. Heuser, T. Berg, M. Copley, J. Ruschmann, S. Sekulovic, F. Antignano, E. Kuroda, V. Ho, C. Benz, T. Halim, V. Giambra, G. Krystal, C.J.F. Takei, A.P. Weng, C. Eaves, J. Piret, M.A. Marra, R.K. Humphries, C.L. Hansen. “Comprehensive microRNA Expression Profiling of the Hematopoietic Hierarchy Using High-Throughput Microfluidic qPCR Arrays”. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES (2010) 107, 15443-15448.
    33. Huft, J., D.J. Da Costa, D. Walker, C.L. Hansen. “Three-dimensional large-scale microfluidic integration by laser ablation of interlayer connections”. LAB ON A CHIP (2010) 10, 2358-2365 
    34. Lam, M.H.C., M.A. Homenuke, C.A. Michal, & C.L. Hansen. “Sub-nanoliter nuclear magnetic resonance coils fabricated with multilayer soft lithography”. JOURNAL OF MICROMECHANICS AND MICROENGINEERING (2009) 19 No 9, Article #095001.
    35. Garanov, A.I., M. Cook, M. Ricicova, G. Ben-Ari, C. Gonzalez, C.L. Hansen, M. Tyers, A. Amon. “The rate of cell growth is governed by cell cycle stage”. GENES AND DEVELOPMENT (2009) 23: 1408-1422. 
    36. R.J. Taylor, D. Falconnet, A. Niemisto, S.A. Famsey, S. Prinz, I. Shmulevich, T. Galitski, and C.L. Hansen. “Dynamic analysis of MAPK signaling using a high-throughput microfluidic single-cell imaging platform”. (2009) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 106, 3758 – 3763. 
    37. A.H. Diercks, A. Ozinsky, C.L. Hansen, J.M. Spotts, D.J. Rodriguez, and A. Aderem. “A microfluidic device for multiplexed protein detection in nano-liter volumes”. (2009) ANALYTICAL BIOCHEMISTRY. 386 (1) 30-5. 
    38. F. Kuchenbauer, R.D. Morin, B. Argiropoulos, O.I. Petriv, M. Griffith, M. Heuser, E. Yung, J. Piper, A. Delaney, A. Prabhu, Y. Zhao, H. McDonald, T. Zeng, M. Hirst, C.L. Hansen, M.A. Marra, and R.K. Humphries. “In-depth characterization of the microRNA transcriptome in a leukemia progression model”. (2008) GENOME RESEARCH (11) 1787-97. 
    39. Hansen, C., Leung, K. & Mousavi, P. “Chipping in to microfluidics”. PHYSICS WORLD 20, 24-29 (2007).
    40. Lau, B. T. C., Baitz, C. A., Dong, X. P., Hansen, C.L., “A Complete Microfluidic Screening Platform for Rational Protein Crystallization”, (2007) JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 129, 454-455 
    41. Hansen, C.L., S. Classen, J.M. Berger, S.R. Quake. “A Microfluidic Device for Kinetic Optimization of Protein Crystallization and In Situ Structure Determination”. (2006) JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. 128.10 (February 2006): 3142 – 3143. 
    42. Anderson M.J., Hansen C.L., Quake S.R., “Phase Knowledge Enables Rational Screens for Protein Crystallization”, (2006) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 103, 16746–16751 
    43. Hansen, C.L., Classen S., Berger J.M., Quake, S.R. “A Microfluidic Device for Kinetic Optimization of Protein Crystallization and In Situ Structure Determination”, (2006) JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 128 (10) : 3142 – 3143. (PDF)
    44. Featured in Science (PDF) and Nature Methods 
    45. M.J. Anderson, C.L. Hansen, and S.R. Quake. “The use of microfluidic devices towards protein crystallography”. (2005) BIOPHYSICAL JOURNAL, Vol. 88 No. 1, 55A.
    46. Balagadde, F.K., You, L., Hansen, C.L., Arnold, F.H. & Quake, S.R. “Long-term Monitoring of Bacteria Undergoing Programmed Oscillations in a Microchemostat”, (2005) SCIENCE, 309: 137-140.
    47. Hansen, C.L., Sommer, M. O. & Quake, S. R., “Systematic investigation of protein phase behavior with a microfluidic formulator”. (2004) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101, 14431-14436. 
    48. Liu, J., Hansen, C. & Quake, S. R. (2003), “Solving the “world-to-chip” interface problem with a microfluidic matrix”. ANALYTICAL CHEMISTRY 75, 4718-4723. 
    49. Hansen, C. L. & Quake, S. R. (2003), “Microfluidics in structural biology: smaller, faster … better”. CURRENT OPINION IN STRUCTURAL BIOLOGY 13, 538-544. 
    50. Hansen, C. L., Skordalakes, E., Berger, J. M. & Quake, S. R., “A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion”, (2002)PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 99, 16531-16536.