Proteomics Core Facility

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The Proteomics Core Facility (PCF) offers a broad range of services, from mass confirmations to large-scale quantitative proteomic screens, along with all the associated bioinformatics. Basically, if it involves characterizing polypeptides, we can probably do it. Some of the services we provide most commonly include:

  1. Measure the mass and identity of a protein or peptide you may have (e.g., to confirm successful synthesis of a peptide or the purity of a recombinant protein
  2. Identify unknown bands from a gel
  3. Characterize post-translational modifications on your favourite protein
  4. Identify the protein interacting partners of a ligand
  5. Measure global changes in protein levels or post-translational modifications (e.g., phosphoproteomics)
  6. Monitor the global interaction landscape of cells

We will work with you to get us the best samples possible and then strive to get the best data on those samples back to you. Please feel free to contact us early in your planning process so that we can advise on how to best design your experiments. In addition, since some large-scale experiments can get quite expensive, we are open to co-applying for grants with you to fund such work. This could be done with the PCF itself as a co-applicant or with Dr. Leonard Foster, the PCF Director.

New in 2014

We are delighted to announce a new service for the research community, through a collaboration with Waters Corp. and Dr. Harry Brumer of the Michael Smith Laboratories. We have implemented a high-throughput system for accurate mass confirmation of purified recombinant proteins aimed at structural biologists or others who commonly have to check the mass of purified proteins by SDS-PAGE. Starting at $15/protein (volume discounts available) we can give you the precise mass of your proteins in as little as one-day turnaround.
 
In addition, the PCF provides training in operation of our ABI Voyager DE-STR MALDI TOF MS through our Long Term Collaborators (LTC) program. In this program, researchers are trained to independently run and obtain MALDI TOF spectra of peptides, proteins and small molecules from their own research labs and have the ability to use the web-based booking system to schedule time on the mass spectrometers. LTC clients are charged hourly rates that are extremely cost-effective. We are also open to collaborative projects on a case-by-case basis. Staff in the PCF is available for project planning and sample preparation in addition to general mass spectrometry scientific advice and consulting on a walk-in basis.
 
 

Leonard Foster

Director, Proteomics Core Facility
University of British Columbia
416-2125 East Mall
Vancouver, BC V6T 1Z4 CANADA
 
Office: 604-822-8311
Fax: 604-827-2603
Email: foster(a)chibi.ubc.ca

Jason Rogalski

Operations Manager, Proteomics Core Facility
University of British Columbia
420-2125 East Mall
Vancouver, BC V6T 1Z4 CANADA
 
Lab: 604-827-5209
Fax: 604-827-2603
Email: rogy(a)mail.ubc.ca