The Proteomics Core Facility (PCF) offers a broad range of services including peptide mapping, database searches, SILAC and isotopic labeling for quantitation analyses, QC of peptide/oligonucleotide and protein samples, metabolomics/small molecule, post-translational modification detection, and enzymatic digestion of protein samples. Our current set of mass spectrometers includes Waters XEVO G2 Synap QTOF, Applied Biosystem’s Voyager DE STR MALDI-TOF, 4700 MALDI TOF TOF, 3 QStar QTOF ESI- LC MS/MSs, Thermo Triple Quad TSQ Vantage, Orbitrap LTQ-XL, LTQ-VELOS and LTQ- FTMS, Agilent 6460 Triple Quad and 6550 QTOF- both with ChipCube interfaces. Analyses on all of these mass spectrometers are available to corporations. We have the capacity to synthesis peptides in-house for the MRM/quantitative MS workflow. On the front end we also can offer computer imaging of 1-D and 2 D gels, Agilent 3100 OffGel liquid phase IEF, Biorad micro ROTOFOR in addition to several off line preparative HPLCs.
In addition, the PCF provides training in operation of our ABI Voyager DE-STR MALDI TOF MS through our Long Term Collaborators (LTC) program. In this program, researchers are trained to independently run and obtain MALDI TOF spectra of peptides, proteins and small molecules from their own research labs and have the ability to use the web-based booking system to schedule time on the mass spectrometers. LTC clients are charged hourly rates that are extremely cost-effective. We are also open to collaborative projects on a case-by-case basis. Staff in the PCF is available for project planning and sample preparation in addition to general mass spectrometry scientific advice and consulting on a walk-in basis.
The CHiBi PCF would not be possible without the generous funding and support from the following: NSERC, CFI, MSL, LSI, BCPN, PENCE, MSFHF, BCKDF, Agilent, Thermo Scientific,Waters Corporation and UBC’s Vice President of Research Office.
For more information about the PCF instruments, prices, support and publications, please follow the links under the left side navigation.
Core Facility Manager:
Suzanne C. Perry, M.S.
Manager and Research Scientist
Proteomics Core Facility
University of British Columbia
420-2125 East Mall
Vancouver, BC V6T 1Z4 CANADA
Phone Office NCE 304: (604) 827-5401 (direct)
Lab 420 NCE: (604) 827-5209
Fax: (604) 822-2114