MISSION shRNA Libraries

SigmaAldrich

MISSIONTM shRNA libraries

The University of British Columbia has recently acquired Sigma’s MISSION shRNA human and mouse whole-genome TRC shRNA libraries that consist of almost 200,000 pre-cloned shRNA vectors targeting more than 22,000 human and 20,000 mouse genes. The MISSION shRNA human and mouse libraries provided by Sigma Life Science and The RNAi Consortium (TRC) were developed at the Broad Institute, a joint venture between MIT and Harvard, and represent the most comprehensive and thoroughly validated shRNA collection available.

 

More information about the library can be found here: MISSION shRNA Information

 

Applications

The shRNA clones could be used in both bacterial plasmid DNA or lentiviral formats to perform robust gene knockdown in a wide range of cells, including non-transfectable and non-dividing cells.

 

Protocols for RNAi using MISSION shRNA products can be found here:

PDFicon Culturing bacterial stocks

PDFicon Lentiviral transduction

PDFicon Preparation of the lentiviral transduction partcles using packaging plasmid mix

 

More information about Sigma’s current shRNA offerings, RNAi technical workshop and Learning Centre can be found here: Sigma shRNA product Information Page

 

Services offered

Whole-genome TRC shRNA libraries, individual shRNA clones, or shRNA gene family sets (gene panels) are available to the University of British Columbia researchers at a cost-recovery price of 10CAD/clone (special discount pricing is offered for gene panels and larger orders). The clones are provided as inoculated 0.5 ml bacterial cultures in tube or plate format for pick-up at the NCE. Technical and reagent assistance is available for larger orders.

 

The searchable list of available shRNA clones can be found here: shRNA Clone Search Tool

 

A batch search tool to create a gene panel can be found here: shRNA Clone Batch Search Tool

 

Please contact Anna Prudova with inquiries about ordering specific shRNA clones from the facility. Please include a list of clone numbers to be ordered.
Anna Prudova
Research Associate
University of British Columbia
405-2125 East Mall
Vancouver, BC V6T 1Z4 CANADA

Lab: 604-827-5209
Fax: 604-827-2603
Email: anna.prudova [at] ubc.ca

 

General inquiries about the MISSION libraries and other Sigma products, applications and for technical support should be directed to Tina Thottingal.

 

Tina Thottingal
Senior Account Development Manager
Academic Research Sales
Sigma-Aldrich Canada
Cell Phone: 778-990-4086
Customer Service: 1-800-565-1400
Email: tina.thottingal [at] sial.com